I groaned in anguish and finally tried to break free and move towards her to stop it but Martha clutched on to me wildly with her arms and legs, bucking her pussy, fucking my cock hard. She continued to suck him until he was ready to fuck her little cunt.Then I saw that black bastard climb on top of my slender wife his huge hardened black cock pointing towards her inviting pink pussy. As we got back we sat down on her couch, and began to make out. As we finished up dinner she incited me back to her place which this time I quickly took her up on her offer. She leaned on my shoulder, as I wrapped my arm around her and as he movie ended she said she was having a great time, and so was I.We went out to the mom and pop diner a few blocks away, it want fancy, but this date and our relationship thus far hadn't been, so I figured she'd be ok with it. Alice snatched off her bra and used that for a tourniquet. The first thing they had to do was to get a tourniquet on the leg before Jeff lost too much blood. Blood was flowing out too fast to be disregarded.Julie had made sure that the girls all had basic training in first aid, and it was a good thing because this was a time when they really needed it. The simple accident turned into a major emergency when the break turned out to be a compound fracture. The first was one that went behind my balls and had a part that went into my ass. I pulled out two object, and cleaned them fast with a cloth and some cleaner, then lubed them up. As she concluded her squirting, I pulled out and walked over to my night stand. I continued my assault on her young pussy. "Oh god! Joshua, that's so deep! I think it's making me pee!" She said as she continued to squirt hard. And while her show was going off without a hitch, things took a turn just before midnight.įrom Teen Mom’s Farrah Abraham to Paris Hilton, there’s been no shortage of stars who flashed a bit too much when striking a left-leg-out pose.įrom Kim Kardashian’s underwear exposure to Jennifer Lawrence’s nip slip, keep scrolling to see the most embarrassing celebrity wardrobe malfunctions of all time.I slammed my cock back in her, making her cry out as she came hard. The singer kicked off 2022 with her NBC New Year’s special. While some stars have froze when faced with a fashion emergency, most of them are able to handle the debacle like a true professional and laugh off the unlucky moment. And most of the time, it’s all going down in the public eye.īecause whether something is snapping on live television or the wind caused a mishap en route to an event, everyone from Lindsay Lohan to Nicki Minaj has found themselves faced with a clothing crisis. Uh oh! From split pants and broken straps to showing a heck of a lot more skin than initially anticipated, stars have had their fair share of cringe-worthy wardrobe malfunctions. Most Embarrassing Wardrobe Malfunctions of All Time: From Broken Straps to Split Pants And more often than not, the wardrobe malfunction is rooted in a thigh-high slit. Live performances complete with moving and grooving may cause some rips and tears in attire, but the red carpet has also seen a fair share of mishaps. Rather than call it quits early, she simply changed into a bathrobe and continued twerking away. “I just wanna let y’all know that my outfit ripped,” she said according to the Tennessean. During a performance at Bonnaroo, the star’s jumpsuit broke down her butt. Lil Nas X’s pants split down the front, but Cardi B had same issue with her backside in June 2019. “I know I do a lot of planned s-t, but ripping my pants on live television is not one of them,” he joked on the social media platform. Following Lil Nas X’s Saturday Night Live Performance in May 2021, during which he completely split his pants during a pole dancing routine, he took to Twitter. “Get a good look at the top that didn’t stay on very long …” she jokingly captioned her post.Ĭyrus isn’t the only star to address their malfunction on social media. She even made fun of the whole shebang after the fact, taking to Instagram to share a picture of her outfit in its glory. Rather than stop singing and scuttle off stage, the Hannah Montana star tossed her shirt aside, covered up her breasts with her arm and strut backstage to change into a blazer. While in the middle of her “Party in the USA” performance, the strap on her top snapped right off.
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Stick with me here because this comes in handy in our next point.Īt my numbers, it will take approximately 30% longer to create the Search campaigns in your account. Hourly Estimate For Separating Match Types It does not take “double” the time to create an account by separating match types out. Now, I understand this isn’t super-duper exact science but you do have to start somewhere, and even if things vary, or some ad groups take you longer/shorter than others, I believe the relationship between the 2 stands. The real time comes from adding the exact KWs as mirrored negatives, and even that is pretty easy with the Editor bulk operation. What I learned was that you actually have to use a similar process to create BM and E keywords, whether in the same ad group or separated so the time saved by grouping them is not as significant as first appears.Īd group, ads, everything else can be copied over to create the duplicate ad groups. (actually I ran this twice, one time it took me 3:30 and the other time it took me 3:00 so I averaged this at 3:15). – mirrored Negative KWs set up in separated MT ad groupġ Ad Group, Both Match Types: 2:15 (135 seconds) From start to account push up to UI.Ģ Ad Groups, Separated Match Types: 3:15 (195 seconds) From start to account push up to UI. – A/B Ad Test set up (copied ads from other ad groups to all AGs) I then created 2 ad groups, for another similar keyword phrase. Using similar keyword modifiers so I was as much as possible comparing apples to apples, I created 1 ad group with both exact and broad modified match types. I went into a real client account, and made a test. Can you spot it? The faulty reasoning is: “Double the ad groups = double the time in account creation.” I was skeptical about this statement because frankly, using Editor, I can blast out multiple ad groups pretty quickly. “In order to create separate Broad Match Modified (BM) and E ad groups, you are in essence DOUBLING the number of ad groups you have to create!” This is an interesting argument since it appears at surface level to hold validity.
Some of these batteries are also designed for crossover use with recreational vehicles (RV). We also offer both lead acid and lithium-ion marine batteries, giving you the ability to choose just the right replacement for your boat. Marine batteries: From small motor boats to large yachts, we offer batteries that can suit a large variety of boat types and sizes in a range of different voltages, amperages and dimensions.You don't have to look anywhere else for your vehicle battery shopping needs. You can a find a variety of battery options for other vehicles, adding convenience and efficiency to your shopping experience. Other optionsĬar and truck batteries aren't the only vehicle battery options available. Many of the batteries and accessories are available with free two-day shipping or same-day in-store pickup so you can quickly get your car or truck back up and running. Walmart's automotive department offers a variety of car battery options in different sizes and voltages in addition to useful battery-related accessories such as portable battery chargers, jumper cables and charging system monitors. Whether you're performing routine maintenance or trying to quickly solve an unexpected battery problem, Walmart has the supplies you need to get the job done at Every Day Low Prices. When your car's battery is old, drained or otherwise not working properly, you may experience problems ranging from a sluggish engine start to leaking battery acid or a "check engine" light on your dashboard. Your car's engine may run on gas but its electrical components, including the headlights, interior electronics and turn signal lights, run off a battery. Stay powered on with essential car batteries Shop all Car Battery Chargers and Jump Starts.Auto Battery Terminals and Battery Terminal Accessories. Both ZAF and ZAF+ are adaptable to any laboratory environment and facilitate the implementation of aquatic colonies. The advanced version, ZAF+, allows for the precise control of food distribution as a function of fish density per tank, and has a user-friendly interface. ZAF is reliable, provides a standardized amount of food to every tank, is cost-efficient and easy to build. We have developed an open-source fully automatic daily feeding system, Zebrafish Automatic Feeder (ZAF). However, a challenging aspect of maintaining aquatic facilities is animal feeding, which is both time- and resource-consuming. Zebrafish are widely studied and relatively easy to culture using commercial systems. And for sure we need to find a better streaming setting that would allow us to watch the stream in better quality.In the past few decades, aquatic animals have become popular model organisms in biology, spurring a growing need for establishing aquatic facilities. It would be helpful to also control the direction of moving and do some shaking when food should fall down. So nothing or just a small portion fell into the water. Yaay again! But there is still space for some improvements for Christmas 2022.įood for the snail is a little bit bigger and sometimes it is stuck with fish food. And because you are reading it, they survived. This article would be most likely private if the fish or the snail would die. If it would not survive, it wouldn’t affect our feeder. So for safety reasons, we used another RapsberryPi just for streaming. The stream was in resolution 640x480px and 15 FPS and it still uses almost 95% of CPU time. So we weren’t able to optimize streaming enough. It is not a big surprise we kept everything to the last days. We have used ffmpeg tool and the prepared script can be found again in our public Gitlab repository.Įveryones know, the developers are lazy. So we decided to do a stream with a web camera on YouTube. But as the feeder is a piece of hardware, it is much harder to monitor. Visual feedback with non-stop stream to YouTubeĪccess with SSH, logging, and monitoring might check the software behavior. You can find the full script in our public Gitlab repository. So even during power strike, fish and snail would be fed after that. And the last feeding time was controlled programmatically. The python script itself was executed regularly every hour by Linux Cron. I was writing about it earlier.And for the worst case, we assigned a static IP address and set up port-forwarding to have access with SSH into the Raspberry. So besides some basic logging into the local file, I set up also monitoring with service Healthchecks.io. I wanted to be sure, it will be working flawlessly. In some situations, I could call myself a control freak. RaspberryOS comes with some nice integration into Python, so controlling GPIO (General-purpose Inputs Outputs) is super easy. We will use only 1 power supply and the feeder will contain also DC/DC step-down module to power Raspberry. And we had an old laptop power adapter that produce 19.5V. But RaspberryPi needs 5V and has 3.3V GPIO pins. The stepper motor driver needs input voltage in the range 8.2 - 45V and a logic voltage between 2.5V and 5.25V. So with colleagues, we decided to use the RaspberryPi which was unused in our office.Īfter we prepared RaspberryPi and we were able to control LED, we realized what will be the next challenge. I spent the whole weekend trying to find another solution to make it work, but unsuccessfully. But they removed also all images required to make the kit alive. I thought it means, that only new features are not coming. However, Google stopped this project meanwhile. I got an Android Things kit during Google Developer Days back in 2017 in Krakow, Poland. So we needed to do the next iteration and print the inner part again with bigger bowls. And the snail’s food was bigger than the bowl in the feeder. The first prototype actually worked! But in the tank, we have also snail, not just fish. First, we had to do a small modification to properly attach stepper motor. But the existing ones are either expensive or not compatible with our aquarium.įor more about side projects in our company, visit /blog So there was no spare tank to move the fish again. But during 2021 that tank became an Aquarium PC. □□ V češtině si lze článek přečíst na īefore Christmas 2020 we used our smaller tank and moved the fish to a colleague's flat. That might be too much for the fish without food. But our office building is closed every Christmas for almost 2 weeks. By that day all of us started to learn how to care about aquarium. All started 2 years ago, when a colleague got a Beta fish and brought it to the office. Do not use chlorine bleach, fabric softeners, or Woolite as these are known to damage or weaken the compression fabric. Add mild soap or detergent - or the cleaning product recommended from the sock manufacturer - to the machine.Set the washing machine on warm, gentle or delicates cycle according to the manufacturer’s instructions.Place the compression socks in a mesh garment bag to protect them during the wash cycle.If there are stains or dirt trapped in the silicone bands, it is advised to treat these areas first.Do not hang garments directly in the sunlight as this may cause fading and discoloration. Remove the garment from the towel and place on a flat surface or hanger and allow to dry over night. Place the stocking on the towel and begin to roll the towel with the garment inside in a snug fashion and allow to sit for a few minutes. Being careful not to wring out your garments take a bath towel and lay on a flat surface.Do not pull, stretch, or twist the stockings, as this can stretch out the material and compromise its proper fit. Finish by rinsing your garments, while gently squeezing the material ensuring the soap is thoroughly removed from your garments.(*We will discuss this below in greater detail). If your garment has a silicone band, additional cleaning may be necessary from time to time to remove the build up from it. Once you feel that your items are clean, pour out the soapy water and repeat step 1 and 2, this time without adding the mild detergent.This process should take no longer than a few minutes. Using your hand, gently move your items through the water and gently message the garments. Place your compression garments into the warm water allowing the garment to soak through.Pour in a small amount of a mild detergent while using your hand to agitate the water ensuring the detergent is evenly distributed. Fill a sink, tub or basin with warm water.Here are the steps to properly clean compression garments, first by hand and then in the washing machine: Compression garments must be washed after each use, and they need to be cleaned carefully to avoid compromising the material. Plant fruit, especially perfect + foreign fruit, which sell for more bells in your town.See this page for more on customizing with ore. These can be sold or used to customize furniture. These will break upon hitting and drop an ore (amethyst, ruby, emerald, sapphire, gold, silver). Every day, a new rock will spawn which is a fake rock.If a bell bag comes out, keep hitting it until no more come out. Stand in front of the holes so that your character doesn't get any knockback from hitting it. Dig 2-3 holes one space away from the rock you want to hit. Every day, a natural rock will become a bell rock.Every town has natural rocks that cannot be moved without hacks. You can catch bees that way by carrying a bug net when you shake trees. When a beehive falls, your character will immediately look over to it. Trees can have bells, furniture, and sometimes bees. A clothing's style will be specified when you try on clothes at Able Sisters or Kicks. Every clothing item also has a specific style.Check out the AC Fandom Wiki page on luck for more information. If not, try to wear different clothes every day. If Katrina the fortune teller is in town, she'll tell you what style to wear to maximize your good luck. There's a hidden game mechanic called luck. Wear different clothing styles every day.Villagers will suggest masculine nicknames. Characters use masculine terms to refer to you. BOY: Selecting "Cool, right?" and "I know, right?" will make the player's gender "boy".Villagers will suggest feminine nicknames. Characters use feminine terms to refer to you. GIRL: Selecting "Cute, right?" and "I know, right?" will make the player's gender "girl".Fortunately gender is not a huge part of the game + clothing and hair are not restricted. GENDER: Cannot be changed without hacks. MAP: The river, plaza, beach orientation, and location of default buildings (Town Hall, Re-Tail) cannot be changed without hacks.If they suggest a nickname for you and you say you don't like it, you can enter a name for them to call you. NAME: Cannot be changed without hacks but you can change what villagers call you.Your hairstyle, hair color, and eye color CAN be changed later. The things you say to Rover will determine what your face looks like. FACE: Cannot be changed without hacks.The things you say will determine aspects of the actual game, and almost everything can't be changed later without hacks. These tips will only be useful if you haven't started a town yet. Villagers: Animal residents of the town.You unlock more over the course of the game. After you become mayor, you can set up PWPs which are things like bridges, street lamps, and benches. PWP: Acronym of "Public Works Project".You can put furniture up in "flea market" spaces for a set price and others can buy your items. Buys your stuff for a higher price than Nook's. Re-Tail / Recycle shop: Shop owned by Reese and Cyrus.Sells tools, furniture, stationery, and special items on holidays. Nookling store / Nook's: The shop owned and ran by Timmy and Tommy. Table of Contents (you can hit Ctrl+F and search for what you need) Your hair style, hair color and eye color can all be changed later at Shampoodle.Reposted this since I made a new reddit account and it's been a while since the original post. You can see each face and the required answers in the video below or as static images on the Face Guide page. However, your responses directly result in which face your character will have - one of 12 possible outcomes. Next, you'll be asked three simple and seemingly unimportant questions. Once you find one you're happy with, you'll be very close to seeing it in person. When asked, "Is this the village you are moving to?", you can have the map regenerated by pressing theīutton or selecting "No." on the touch screen. In a new feature to New Leaf, you will then be shown a map of what it will look like. You will be asked during the train ride to name your town. He'll give you one last chance to confirm before you're stuck that way forever. Next, choose your gender by declaring your name "cool" for male or "cute" for female. Feel free to type whatever you'd like, but remember that this is what you'll be known as for the rest of the game. After confirming the time, Rover will ask for your name. Modified BSD: jersey-inject-hk2, jersey-media-jaxb, jersey-media-json-jettisonĮclipse Distribution License - v 1.0: Jakarta XML Binding APIĮPL 2.0: HK2 API module, HK2 Implementation Utilities, Jakarta Annotations API, OSGi resource locator, ServiceLocator Default Implementation, aopalliance version 1.0 repackaged as a module, .rs-api, javax. W3C license: jersey-inject-hk2, jersey-media-jaxb, jersey-media-json-jettisonĮDL 1.0: Jakarta Activation API jar, jersey-inject-hk2, jersey-media-jaxb, jersey-media-json-jettison Public Domain: jersey-core-common, jersey-inject-hk2, jersey-media-jaxb, jersey-media-json-jettison GPL2 w/ CPE: HK2 API module, HK2 Implementation Utilities, Jakarta Annotations API, OSGi resource locator, ServiceLocator Default Implementation, aopalliance version 1.0 repackaged as a module, .rs-api, javax.inject:1 as OSGi bundle, jersey-inject-hk2, jersey-media-jaxb, jersey-media-json-jettison JQuery license: jersey-inject-hk2, jersey-media-jaxb, jersey-media-json-jettisonĮclipse Public License v2.0: JUnit Jupiter (Aggregator), JUnit Jupiter API, JUnit Jupiter Engine, JUnit Jupiter Params, JUnit Platform Commons, JUnit Platform Engine API The Apache License, Version 2.0: org.apiguardian:apiguardian-api, org.opentest4j:opentest4j Project License: MPL 1.1 LGPL 2.1 Apache License 2.0Īpache License, 2.0: jersey-core-common, jersey-inject-hk2, jersey-media-jaxb, jersey-media-json-jettison It is a class library for editing bytecodes in Java. :jersey-media-json-jettison:jar:2.38ĭescription: Javassist (JAVA programming ASSISTant) makes Java bytecode manipulation Latest Stable: 1.5.4.jar Latest Release Candidate: 1.0-RC2.jar Latest Beta: 1.0-beta-1.jar Latest Alpha: 1.0-alpha-1.jar All Versions Download : jettison JAR file - All Versions: View Java Class Source Code in JAR file Download JD-GUI to open JAR file and explore Java source code file (.class.Transitive dependencies are the dependencies of the project dependencies. The following is a list of transitive dependencies for this project. These dependencies are only required to compile and run unit tests for the application: GroupId The following is a list of test dependencies for this project. These dependencies are required to compile and run the application: GroupIdĮPL 2.0- The GNU General Public License (GPL), Version 2, With Classpath Exception- Apache License, 2.0- Public DomainĮPL 2.0- GPL2 w/ CPE- EDL 1.0- BSD 2-Clause- Apache License, 2.0- Public Domain- Modified /license- MIT license- W3C license The following is a list of compile dependencies for this project. It has been reported that during carcinogenic transformation in mammals, severe changes in normal glycosylation occurred. LB is commonly used for detection of protein glycosylation based on lectin–glycan interaction 7. IB is widely used to detect and semi-quantify antigens, determine the relative molecular weight of polypeptide chains and the extraction efficiency of target antigens 6. The process can be described simply as first separating natural or denatured proteins by gel electrophoresis, then transferring the isolated protein bands on the gel to the solid phase supporting material, followed by immunoblotting (IB) and lectin blotting (LB), respectively. Since its inception in 1979, Western blotting (WB) has become a routine experimental method for the determination of proteins and their expression levels in molecular biology and proteomics, as well as used to study protein abundance, kinase activity, cellular localization, protein–protein interaction or monitor protein post-translational modification 5. Therefore, the detection of target protein and changes of abnormal glycosylation are extremely vital for the exploration of new biomarkers for certain diseases. In addition, alpha-1-acid glycoprotein (AGP) as a protein can be used as a prognostic marker of lung cancer 3, and a diagnostic marker for laryngeal cancer 4. Human epidermal growth factor receptor 2 (HER2) is an important indicator for molecular typing of breast cancer, and glycoantigen 19-9 (CA19-9) is used as an important indicator to judge the prognosis of pancreatic cancer 2. What’s more, with the development of proteomics and glycobiology, some proteins and glycoproteins in peripheral blood have become routine tests for diagnosing diseases and predicting prognosis in clinic, such as Alpha-fetoprotein (AFP), a core fucosylated protein used in the diagnosis of liver cancer. Glycoproteins play an important role in cell signaling, immune recognition and cell–cell interaction 1. More than half of the proteins in eukaryotic cells are glycoproteins. Glycosylation is a common post-translational modification of proteins. Protein is the basis of life, which is not only the key component of cell, but also the main executor of biological functions. Bound proteins were stained with direct blue-71, and the staining intensity was quantitated by scanning and densitometry. Moreover, we verified the above results by directly incubating proteins having different molecular weights onto PVDF or NC membranes. The antibody re-probed ability of the two membranes was also explored. Here, by incubating proteins which were transferred to PVDF or NC membranes with a series of antibodies and different types of lectins, we investigated the relationship between the binding ability of these two membranes to proteins or glycoproteins and the molecular weight of the target protein. Which membrane of these two is more suitable for WB has not been reported so far. The most common used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes. The selection of electroblotted membrane is one of the key factors affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. Western blotting (WB) is one of the most widely used techniques to identify proteins as well as post translational modifications of proteins. This is all greatly supported by having an excellent voice acting cast who did a superb job of bringing the team to life. What I liked best about these little moments was that they were really insightful in showing the sacrifices researchers have to take when they go away from their home life on these expeditions, whilst also helping to flesh out the personalities of each of the research team members too. These interactions give you a few alternative dialogue choices, however, they don’t influence the game’s story. What I wasn’t expecting from this underwater exploration game was that between dives you get to spend time on Mirai’s submarine base, where you get snippets of interactions with her research team members, Andre and Irina, as well as, learning about her family life through communication with her sister, Ren. Whilst on this journey, Mirai documents the sea creatures she finds throughout the varied ecosystems she explores in the format of live streams to the ‘home audience’. What sets Beyond Blue apart from other recent marine-life exploration titles, such as the open-world survival game Subnautica or the rather beautifully artistic game Abzu, is the sheer attention to detail in providing a realistic and educational oceanic adventure game, that makes it feel a lot closer to the classic Wii game Endless Ocean which released over a decade ago.īeyond Blue’s narrative follows Mirai’s main objective of researching a family of sperm whales whilst also touching on ocean conservation issues with the consequences of deep-sea mining. The game places you in the shoes, or should I say flippers, of marine biologist Mirai who is the lead researcher working for the OceanX initiative, the actual research and expedition team that helped fund the game, tasked with charting the waters of the South China Sea. As mentioned in the article, I have a science and research background with a keen interest in nature so instantly, the premise of Beyond Blue appealed to the explorer and researcher in me.Ĭo-created with BBC Studios and based on the hit BBC series Blue Planet II, Beyond Blue is a single-player narrative-driven exploration game set in the near future. At the time I was fortunate enough to preview the game and interview Steve Zimmermann, Vice-President of Marketing at E-Line Media, which you can find HERE. Beyond Blue, from developer E-Line Media, was the first game to catch my attention at the gaming convention EGX back in 2018. “If an officer has violated policy or the law, if they have, they will be held accountable.” The Ohio Bureau of Criminal Investigation “will conduct a fully independent investigation, which will be made public,” Pettus said. “Fast, quick answers cannot come at the cost of accurate answers,” Pettus told reporters, appearing alongside Woods and Columbus Mayor Andrew Ginther. Ned Pettus Jr, the city’s public safety director, appealed for calm while the investigation proceeds and facts are uncovered in what he called “a devastating” loss of life. The chief did not identify the officer who shot her but said he “would be taken off the street” pending the inquiry. Woods said investigators will determine whether the killing complies with state law, which says “deadly force can be used to protect yourself or the protection of a third person”. But family members have identified her as Makiyah Bryant, aged 16. It was not clear whether anyone else was injured.Īuthorities said the girl who was fatally shot was 15 years old. The girl was taken to a hospital, where she was pronounced dead, police said. A woman reacts as investigators work at the scene where Makiyah Bryant was shot and killed by a police officer in Columbus, Ohio, US, Ap A crowd gathers to protest in the neighborhood where a Columbus police officer fatally shot a teenage girl Tuesday, April 20, 2021, in Columbus, Ohio. The video then shows what appears to be a kitchen knife lying on the pavement near the teenager. Police arriving at the home encountered a chaotic scene of several people on the front lawn of a house where the girl, seen brandishing what appeared to be a knife, was charging towards another person who fell backwards, the video showed.Ī police officer then opened fire on the girl as she collapsed against a car parked in the driveway. Keep reading list of 4 items list 1 of 4 Derek Chauvin found guilty of murdering George Floyd list 2 of 4 Police did not get Daunte Wright swift medical care, witness says list 3 of 4 Police chief, officer resign two days after Black motorist killed list 4 of 4 US police officer accused of assaulting Black Army officer fired end of listĪt a news conference in Columbus, police released body-camera footage of the latest shooting and the city’s interim police chief, Michael Woods, said officers involved in the incident were answering an emergency call from someone who reported an attempted stabbing. |
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